HCMUS - Danh sách bài trích tạp chí (The List of Article)
1684 ấn phẩm có sẵn
Đang tải...
Tóm tắt
Cách thức đăng nhập
Điều kiện đăng nhập:
- Bạn đọc đã đăng ký sử dụng thư viện;
- Bạn đọc thuộc nhóm đối tượng phục vụ tại Thư viện;
- Bạn đọc tuân thủ quy định của bộ sưu tập số.
Cách thức đăng nhập:
- Đối với sinh viên:
Username: 12000+[Mã số sinh viên] - Đối với học viên cao học và nghiên cứu sinh:
Username:12000+3+[Mã số học viên/Nghiên cứu sinh--Bỏ chữ cái (N/C)] - Đối với Cán bộ-Giảng viên:
Username:12000+9000+[Mã số cán bộ--Đầy đủ 4 chữ số]
Những tài liệu tải lên gần đây
- Ấn phẩmEnhanced antioxidant metabolites and enzyme activities in Vietnamese Ngoc Linh ginseng Panax Vietnamensis Ha et Grushv) induced by yeast extract(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2026-06) Nguyen, Van Phuong; Pham, Ngan Anh; Dinh, Xuan Tu; Huynh , Thi Phuong Linh; Mai, Thi Phuong NgaNgoc Linh ginseng (Panax vietnamensis Ha et Grushv.) is a valuable medicinal plant known for its richness in ginsenoside saponins and exceptional pharmacological properties. However, excessive harvesting in the wild has led to serious depletion of natural populations and raised significant conservation concerns. As a result, alternative strategies are needed to ensure its sustainable use and preservation. In vitro cultivation has emerged as a sustainable approach to enhance bioactive compound production. This study investigated the effects of yeast extract (YE) elicitation at concentrations of 0.1 g/L (YE1) and 0.2 g/L (YE2) on antioxidant enzyme activity and secondary metabolite accumulation in P. vietnamensis to improve cultivation efficiency and phytochemical yield. Results revealed that, regarding antioxidant enzyme activities, YE1 significantly boosted catalase (CAT) activity, while YE2 increased peroxidase (POD) activity, suggesting distinct oxidative stress responses of these two enzymes towards yeast extract elicitation. In terms of chemical compound production, gallic acid, aescin, and quercetin were used as standard compounds to build the standard curve in phytochemical measurement. The results showed that YE1 increased total phenolic content (25 mg gallic acid-equivalent/g extract) and total saponin content (>600 mg aescinequivalent/g extract), while both YE1 and YE2 significantly increased total flavonoid content (56–57 mg quercetin-equivalent/g extract) compared to the control. A strong positive correlation between the total saponin, total flavonoid, and total phenolic content was obtained, with correlation coefficients of r = 0.96, r = 0.63, and r = 0.83, respectively. Therefore, yeast extract is considered a promising biological elicitor to enhance the phytochemical potential and the sustainable cultivation of Vietnamese Ngoc Linh ginseng.
- Ấn phẩmStudy on culture conditions to enhance the production of α- glucosidase inhibitor, acarbose, from Actinoplanes hulinensis 1094(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2026-06) Nguyen, Thi Trung; Nguyen, Nhat Linh Nguyen; Luu, Minh Duc; Le,Thanh Hoang; Nguyen, Thi Anh Tuyet; Tran, Thanh Tuan; Pham, Duy Nam; Do, Thi TuyenAlpha−glucosidase inhibitors are a class of drugs that are widely used in the treatment of type 2 diabetes mellitus. Members of this class, such as acarbose, are pseudo−oligosaccharides that act as a competitive α−glucosidase inhibitors and have been widely used in the treatment of type 2 diabetes due to thier ability to regulate postprandial blood glucose levels. They competitively inhibit enzymes that convert complex non−absorbable carbohydrates into simple absorbable carbohydrates. By delaying carbohydrate absorption, they reduce the rise in postprandial blood glucose. Acarbose has been shown to increase life expectancy in patients with type 2 diabetes mellitus and reduces the risk of the development of cardiovascular events in patients with impaired glucose tolerance. In this study, we aimed to enhance the biosynthesis of acarbose by optimizing the fermentation conditions of Actinoplanes hulinensis 1094. In 7 surveyed media (MT1, MT2, MT3, MT4, MT5, MT6, and MT7), the α−glucosidase inhibitory activity was highest in MT7 medium, the activity was 58.6%, the lowest was in MT6 medium. Component optimization of MT7 led to the formulation of an optimal medium containing (g/L): 50 maltose, 15 corn flour, 1 monosodium glutamate, 2 CaCl2, 0.5 FeCl3, 1.0 K2HPO4, and 2.5 CaCO3. Subsequent optimization of physical conditions identified 30°C, pH 7.0, and 200 rpm as the most favorable parameters. Under these optimized conditions, the α−glucosidase inhibitory activity reached 82.2%, surpassing that of the 1 mg/mL standard acarbose control. These findings highlight the potential of A. hulinensis 1094 as a promising strain for high−yield acarbose production under cost−effective and scalable conditions.
- Ấn phẩmThe first success in utilizing the RUBY reporter gene for rice transformation in Vietnam(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2026-06) Pham, Thi Mai Anh Pham; Ly, Khanh Linh Ly; Chu, Khanh Linh Chu; Phan, Quyen; Bui, Phuong Thao; Le, Hong Diep; Nguyen, Xuan Cuong; Chu , Hoang Ha Chu; Do, Tien PhatTo date, several reporter genes have been developed, such as GFP, GUS, and luciferase. However, GFP and luciferase genes are costly and require modern equipment, while GUS requires sample destruction. To overcome these limitations, the RUBY gene is considered a promising alternative. RUBY is an artificial open-reading frame, encoding CYP76AD1, LDOPA 4,5-dioxygenase, and glucosyltransferase, which are key enzymes involved in the biosynthesis of betalain, which displays a red-colored pigment, providing a visual marker for transformation events. In this study, the RUBY gene was successfully transformed into the model rice variety Kitaake via the Agrobacterium-mediated method. The activity of the RUBY gene was visually detected by red spots in calli and somatic embryos through the rice transformation and regeneration procedure. Moreover, the presence and expression of this reporter gene were confirmed by PCR and the red coloration in the regenerated rice lines. The RUBY gene was stably expressed in transgenic rice lines under both in vitro and greenhouse conditions and was heritable in the T1 generation, without exhibiting any adverse effects on plant growth and development. This result demonstrates the potential of RUBY as a visual selection marker for rice transformation in Vietnam and opens the door to further applications in other important crops.
- Ấn phẩmA clinical, genetic, and treatment analysis of two Vietnamese patients with vitamin D-dependent rickets type 1A(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2026-06) Tran, Thi Anh Thuong; Nguyen, Ngoc Lan; Vu, Chi Dung; Can, Thi Bich Ngoc; Nguyen, Thi Thuy Hong; Nguyen, Van Tung; Nguyen, Thi Kim Lien; Nguyen, Thanh HienThis study examined the clinical and genetic features of two unrelated Vietnamese families exhibiting the characteristic symptoms of vitamin D-dependent rickets type 1A (VDDR-1A). The affected patients presented with prominent skeletal deformities, severe hypocalcemia, and markedly elevated serum levels of alkaline phosphatase (ALP) and parathyroid hormone (PTH), despite having normal or elevated concentrations of 25-hydroxyvitamin D [25(OH)D]. Genetic analysis revealed a homozygous frameshift mutation (c.1319_1325dupCCCACCC; p.Phe443Profs*24) in both patients. This previously reported pathogenic variant follows an autosomal recessive inheritance pattern. In both families, the asymptomatic parents were confirmed to be heterozygous carriers, and the patients’ siblings carried the mutation in a heterozygous state but exhibited no clinical manifestations. This mutation appears to result in early-onset and severe skeletal abnormalities, consistent with the phenotype observed in our patients. Treatment with calcitriol led to significant clinical and biochemical improvement over time, including normalization of calcium and PTH levels and a reduction in skeletal symptoms. Continued follow-up has demonstrated sustained improvement, highlighting the efficacy of targeted therapy in genetically confirmed cases.
- Ấn phẩmCloning and in silico characterization of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase from Bacillus subtilis(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2026-06) Nguyen, Quang Huy; Nguyen, Thi Thanh Xuan; Nguyen, Minh Thu; Chu, Luong Luan3-Hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI) are key enzymes of the ribulose monophosphate (RuMP) pathway, which plays an essential role in formaldehyde assimilation and detoxification in methylotrophic microorganisms. Although homologous genes encoding these enzymes are annotated in the genome of Bacillus subtilis, their molecular and structural characteristics remain poorly explored in this organism. In this study, taxonomic identification using Kraken2, 16S rRNA phylogenetic analysis, and multilocus sequence typing confirmed the strain as B. subtilis ST123. Moreover, the hxlA and hxlB genes encoding HPS and PHI were cloned from a local B. subtilis ST123 strain isolated from infant fecal samples. The amplified hxlA (633 bp) and hxlB (558 bp) genes were successfully cloned, sequenced, and analyzed. Sequence alignment revealed high conservation with reference B. subtilis sequences. The three-dimensional structure of HPS was predicted using AlphaFold3 and rigorously evaluated by MolProbity, ERRAT, Verify3D, and ProSa. The HPS model exhibited high overall structural quality, with a MolProbity score of 1.13, 100% residues in the Ramachandran favored region, and an ERRAT quality factor of 99.505, although localized regions of lower sequence-structure compatibility were identified by Verify3D. The PHI structure was analyzed based on an available X-ray crystal structure (PDB ID: 1M3S). Physicochemical characterization indicated that HPS is thermostable and suitable for heterologous expression, whereas PHI showed a higher instability index, suggesting potential challenges during in vitro expression. This work provides the first comprehensive cloning and in silico characterization of HPS and PHI from a Vietnamese B. subtilis strain, offering valuable insights for metabolic engineering, synthetic biology, and C1 assimilation pathway design.
- Ấn phẩmAn antibiotic phenotype and genotype assessment of Aeromonas spp. isolates from striped catfish with motile Aeromonas septicemia in Vietnam(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2026-06) Truong, Nhat Ha Minh; Phan, Nam Hoang; Le, Vy Tran Thao; Vo, Phuong Hong; Le, Phuoc Hong; Pham, Thanh Duy; Nguyen, Duc HoangMotile Aeromonas Septicemia (MAS), caused by Aeromonas hydrophila and Aeromonas dhakensis, is a major disease affecting striped catfish (Pangasianodon hypophthalmus) aquaculture in the Mekong Delta, Vietnam. The overuse of antibiotics to treat MAS disease in catfish farming has contributed to the emergence of antimicrobial resistance (AMR) in these bacterial species. This study aims to provide an insight into antibiotic susceptibility in Aeromonas spp. associated with MAS in striped catfish. Forty-four isolates were analyzed through disk diffusion susceptibility testing and genomic analysis for antibiotic gene, plasmid and integron detection. A phylogenetic tree was also constructed to examine resistance patterns and their associations with epidemiological metadata. Results showed that A. hydrophila, A. dhakensis, A. veronii, and A. enteropelogenes exhibited multidrug resistance, especially to sulfonamide-trimethoprim, tetracycline, and norfloxacin. Genomic analyses revealed antibiotic resistance in genes across Aeromonas spp. except for A. salmonicida and A. rivipollensis, with statistical tests confirming significant associations between sul12, dfrA1 and resistance to STX and tetA/R to tetracycline resistance, respectively. Class 1 integrons containing resistance cassettes were also found exclusively in A. hydrophila, whereas A. dhakensis carried diverse plasmid determinants. Phylogenetic reconstruction grouped isolates into five species-specific clades of (1) A. hydrophila, (2) A. dhakensis, (3) A. veronii, (4) A. enteropelogenes, and (5) A. salmonicida and A. rivipollensis; each displaying distinct resistome signatures and regional clustering. These findings indicate that the intensifying use of antibiotics in pangasius aquaculture accelerates AMR development in both species through different genetic pathways. This study provides important insights into antibiotic resistance capability and potential dissemination mechanisms of different Aeromonas spp., thus urging the need for improved antibiotic stewardship and surveillance under a One Health approach.