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  • Authors: Kandil, Mahrous Mahmoud (2006)

  • Cyanuric acid is a key intermediate in the biodegradation pathway of atrazine which has been detected in ground and surface waters because of its widespread use around the world. Few strains are known for their ability to degrade and mineralize atrazine. Some of these strains harbor the atzD and trzD genes, which encode for cyanuric acid amidohydrolase, essential for cyanuric acid mineralization into carbon dioxide and ammonia. For more than 20 years, research has revealed that urea is one of the intermediates in cyanuric acid degradation. However, recent studies suggest that urea is not produced during the metabolism of atrazine or cyanuric acid. In this thesis, hundreds of isolates capable of degrading cyanuric acid were isolated and purified using direct plating method and a cle...