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Sử dụng ngà răng người được xử lý làm giá thể cho tế bào gốc tủy răng tạo mô dạng ngà = Using treated human dentin as a scaffold for dental pulp stem cells to generate dentin-like tissue / Trần Lê Bảo Hà chủ nhiệm ... [và những người khác].
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Tóm tắt
Regeneration of dentin tissues in the pulp
space of teeth serves the ultimate goal of preserving
teeth via endodontic approaches. In recent times,
many studies suggested that human dentin scaffolds
combined with dental stem cells was a potential
strategy for the complete dentin tissue regeneration. In
this study, human dental pulp stem cells (DPSCs) were
isolated and cultured. Dentin specimens were prepared
from human third molars and treated with ethylene
diamine tetra-acetic acid and citric acid to remove the
smear layer. Then, DPSCs were cultured onto human
treated dentin (hTD) and implanted in mouse model
for 4, 6 and 8 weeks. The resulting grafts were
assessed by hematoxylin and eosin stain and immunohistochemical stains. As a result, DPSCs were
supported and induced to regenerate of dentin-like
tissues which expressed specific dentin markers such
as dentin sialophosphoprotein and dentin matrix
protein 1 by combination with hTD in vivo. Furthermore, cells existed in the newly-formed dentin-like
tissues also expressed typical human mitochondria
antibodies, demonstrated that new tissues originated
from human. In conclusion, the obtain results extend hopefully newly-established therapy to apply in
endodontics and traumatic dental hard tissues.
Mô tả
66 tr.
Tác giả
Trần, Lê Bảo Hà, PGS.TS.
Người hướng dẫn
Nơi xuất bản
Nhà xuất bản
Trường Đại học Khoa học Tự nhiên
Năm xuất bản
2015
ISSN tạp chí
Nhan đề tập
Từ khóa chủ đề
Dentistry. , Nha khoa.