Ấn phẩm:
Blockade of interleukin-33 activities by recombinant interleukin-33 Trap Fc protein would be a novel therapeutic strategy in allergic asthma
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The majority of autoimmune and allergic diseases are associated with abnormal expression of interleukin (IL)-33, a member of the IL-1 family of cytokines, that function dually as a proinflammatory cytokine and a transcriptional factor. We created an IL-33 inhibitor called "IL-33 Trap Fc" constructed by fusion of an Fc fragment of human immunoglobulin G1 and two distinct extracellular part receptors involved in interacting with IL-33, IL-1 receptors accessory protein, and IL-33 receptor. IL-33 Trap Fc was expressed by two systems, mammalian HEK293 cells and Pichia pastoris yeast. We found that these recombinant proteins were expressed as a glycoprotein and perhaps in dimeric form. IL-33 Trap Fc from HEK293 and P. pastoris suppressed the activity of IL- 33 in vitro culture conditions. The glycosylation of IL-33 Trap expressed by P. pastoris yeast was more intensive and heterogeneous than the counterpart protein expressed from HEK293 cells. That is maybe one reason leading to a substantial decrease in the activity of IL-33 Trap Fc from P. pastoris compared with that from HEK293 cells. We also demonstrated that IL-33 Trap Fc expressed from HEK293 cells had therapeutic effects in ovalbumin-induced asthma mouse model. These data collectively suggested that IL-33 Trap Fc potently blocks IL-33 in vitro and in vivo, which may be a novel therapeutic strategy for IL-33-mediated allergic diseases.
Mô tả
Vietnam Journal of Biotechnology, Vol. 20, No. 3
Tác giả
Nguyen, Thi Thanh Thao
Vo, Hong Phuc
Nguyen, Dang Quan
Người hướng dẫn
Nơi xuất bản
Nhà xuất bản
Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
Năm xuất bản
2022-09
ISSN tạp chí
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Từ khóa chủ đề
Interleukin-33 , Interleukin-33 receptor , Allergic asthma mouse model
URI
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Thao Thi Thanh Nguyen, Phuc Hong Vo, Quan Dang Nguyen.pdf
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