Browsing by Author Williamson, Kurt Elliott
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The importance of viruses in aquatic ecosystems has been established over the past decade. By contrast, the abundance and distribution of viruses in soils is almost completely unknown. An essential first step in any investigation of viruses in soil is evaluation of viral recovery methods suitable for subsequent cultivation-independent analyses. A comparison of four common elution buffers indicated that potassium citrate eluted the highest numbers of viruses from soils (1.5 X 108 gram dry weight-1 grand mean), but specific soil-eluant combinations posed significant problems to enumeration by epifluorescence microscopy. Thus, the measurement of phage abundance in soils varied with both ...