04. Trường Đại học Khoa học tự nhiên (University of Science)
Tài nguyên nội sinh
Các bộ sưu tập số Luận văn Thạc sĩ, Luận án Tiến sĩ, Đề tài Báo cáo khoa học… của Thư viện Trường ĐH. Khoa học tự nhiên – ĐHQG-HCM được xây dựng nhằm hỗ trợ cho hoạt động giảng dạy và nghiên cứu của Nhà trường.
Duyệt 04. Trường Đại học Khoa học tự nhiên (University of Science) theo Chủ đề "16S rRNA"
- Ấn phẩmDiscovery of Brevibacterium predominating in fecal samples from three children with persistent diarrhea negative for common pathogens(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2024-09) Nguyen, Thi Quy; Dao, Trong Khoa; Pham, Thi Thanh Nga; Phung, Thi Bich Thuy; Nguyen, Minh Hang; Nguyen, Thi Viet Ha; Do, Thi Huyenn this study, to investigate dominant bacteria in stool samples of three persistent-diarrheal children with unidentified pathogenic agents, the V3 and V68 regions of the 16S rRNA gene were amplified from fecal bacterial metagenomic DNA, separated on DGGE gel, and the dominant DNA bands were sequenced. As a result, the V3 and V68 regions of bacteria in persistent diarrheal children were less diverse and different from the corresponding DNA bands of the indicator strains. Sequence analysis and similarity comparison of six DNA bands of V3 regions and seven DNA bands of V68 regions showed that two V3 sequences (of 160 bp) derived from two samples were novel and did not match any genes from the non-redundant database, but they shared 93.75% similarity to each other.
- Ấn phẩmIsolation and characterization of Salmonella enterica associated with diarrhea in chickens and ducks in Hai Duong province(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2024-09) Dong, Van Quyen; Pham, Thi Lanh; Nguyen, Kim Oanh; Tran, Nam KhangThis study focuses on isolating and characterizing Salmonella strains associated with diarrhea in chickens and ducks in Hai Duong province, Vietnam. The Salmonella strains were initially isolated using a culture-based method, followed by identification and characterization through Matrix-Assisted Laser Desorption Ionization/Time of Flight (MALDI-TOF), PCR amplification of the invA gene, and 16S rRNA gene sequencing. As a result, 18 Salmonella isolates were obtained, all of which contained the invA gene, indicating its potential significance in Salmonella pathogenesis. Analysis of the 16S rRNA gene sequences confirmed that all isolates belonged to the species Salmonella enterica, a well-known causative agent of intestinal diseases in humans and animals. Furthermore, phylogenetic analysis revealed that all 18 isolates grouped with Salmonella enterica subsp. enterica strains from China and Korea, suggesting a close relationship with strains circulating in the broader Southeast Asian region. This regional similarity may be attributed to the movement of poultry and poultry products, facilitating the cross-border spread ofSalmonella.
- Ấn phẩmMetagenomic characterization of archaeal and bacterial communities associated with coral, sediment, and seawater in a coral reef ecosystem of Phu Quoc island, Vietnam(Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2023-12) Nguyen, Thi Phuong Thao; Vu, Minh Ngoc; Pham, Van Tra; Bui, Van NgocThe data collected were sequenced using the 16S rRNA sequencing method and further analyzed using bioinformatics tools in the R programming language, employing DADA2 and phyloseq pipelines. We examined the compositions and diversity of bacteria and archaea in coral, sediment, and water column samples to establish potential connections between these two domains. The results revealed that archaea constituted a small percentage of all samples, averaging 3.18% in coral mucus and reaching an average of 7.49% in sediment samples. Among the most abundant archaeal taxa were Crenarchaeota and Nanoarchaeota, alongside bacterial taxa Gammabacteria, Cyanobacteria, and Desulfobacteria, which are associated with important metabolic processes within coral hosts. Alpha and beta diversity analyses confirmed the highest archaeal diversity in sediment samples and the distinct existence of microbial communities in each biotope. These findings complement our knowledge of archaea’s presence and potential roles in the coral-associated microbiome.
- Ấn phẩmScreening of actinomycetes from Ninh Thuan and Binh Thuan sea for antimicrobial producers(Viện Hàn lâm Khoa học và Công nghệ Việt Nam, 2022-12) Vu, Thi Thu Huyen; Le, Thi Hong Minh; Nguyen, Mai Anh; Vu, Thi Quyen; Doan, Thi Mai Huong; Pham, Van CuongThe sea is a unique ecosystem for finding secondary metabolites from marine microorganisms. Many compounds produced by marine microorganisms with antibacterial activity have been applied in life. Furthermore, microorganisms are renewable raw materials and media for drug production, thus crucial for sustainable development in our life. In this study, we screened for antibacterial activity of microbial strains isolated from tern samples from Ninh Thuan and Binh Thuan seas. The antibacterial activity of the marine microbial crude extracts was performed by the Bioassay method in a 96-well tray. The minimum inhibitory concentration (MIC) test results showed that from 86 strains of microorganisms isolated from the waters of Ninh Thuan and Binh Thuan, 68 strains were able to inhibit the growth of 1 to 6 strains of the tested microorganisms, and 27 over 86 strains had antibacterial activity against at least three strains of microorganisms tested. In which strains G631, G756, G769, and G778 inhibited 4 to 6 tested microorganisms with MIC values equal to or lower than positive controls. Morphological characteristics and molecular biological analysis identified the four most active strains. The results showed that all four strains were actinomycetes.