Molecular biotechnology laboratory manual

Abstract

Molecular biotechnology is applied course, which is developed from the basic of many research areas such as molecular biology, microbiology, biochemistry, immunology, genetics, and cell biology. The laboratory techniques are used study and modify nucliec acids and proteins for applications in areas such as human and animal health, agriculture, and the environment. By the molecular biotechnology techniques people have ability to transfer genetic information between organisms and creating the useful products. Molecular biotechnology is a rapidly changing and dynamic filed along with the Human Genome project. The importance and impact of molecular biotechnology is being felt across the nation. To demonstrate the useful of molecular biotechnology subject in the biotechnology files, beside the theory, the labwork provides the basic techniques to treat the nucleic acid molecules and cell also. In which, the technique to isolate genetic material from organisms, create the recombinant ADN, transformation recombinant ADN into the host cell, culture cell, prepare host cell and select the target cells also identify the recombinant ADN plasmid… will be performed. In each practical student will learn the skill to perform and also learn the whole cloning process through the course with seven practicals.